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doi: 10.1016/j.chnaes.2020.02.001 78

In IDH1 mutant glioblastoma, BCAT1 is transcriptionally suppressed because of the hypermethylation of three CpGs in the promoter, and this is also the consequence of IDH1 mutation-induced 2-hydroxyglutarate (2-HG) (Tonjes et al., 2013)

For each spindle pole measurement, this ROI was moved to a nearby area of the cell, not covering the spindle/spindle pole, and a background intensity measurement was obtained