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The autosampler was held at 4C, and 4 L of each sample was injected per analysis

Reconstitution Protocol When preparing the blend for analytical use, introducing a bacteriostatic or sterile liquid agent must be handled with care: Allow the vial to naturally reach room temperature before introducing liquids to prevent thermal shock to the peptide chains

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4.8 Zinc Zinc concentration is mainly regulated by fecal excretion, which includes both unabsorbed dietary zinc and pancreatic, biliary, and intestinal secretions (enterohepatic cycling)