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By reducing lipid hydroperoxides in the presence of ferrous iron, Fer-1 prevents lipid membrane damage and exerts potent anti-ferroptotic effects ( T4, a G-actin sequestering peptide, plays a pivotal role in regulating actin polymerization and is implicated in a wide range of critical biological processes, including cell migration, wound healing, and inflammation ( GB, a terpenoid compound derived from Ginkgo biloba leaves and root bark, is one of the key bioactive constituents known for its therapeutic properties ( Lip-1, a spiroquinoxaline derivative, exhibits potent and selective inhibitory effects on iron-dependent lipid peroxidation pathways, making it a promising candidate for targeting ferroptosis

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For all experiments involving stable isotope tracers, including 1- 13 C-glucose, 1,2- 13 C-glucose, U- 13 C-glucose and 3- 2 H-glucose (all from Cambridge Isotope), isotopically labelled glucose was substituted for unlabelled glucose at the same concentration in RPMI culture medium

Alternating between these approaches provides continuous fat loss support while respecting the need for receptor recovery