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Negative result : Indicates no detectable cotinine in the urine, suggesting no recent nicotine exposure

Although no crave episode frequency studies are reported for oral tobacco users, the average nicotine smoker reports a peak of six crave episodes on day 3

This does happen, but the nicotine levels are much lower than a typical tobacco user

Both linkage studies and genome wide association studies (GWAS) have identified candidate genes/genomic regions associated with nicotine dependence ( Genetic factors significantly influence both smoking initiation and persistence, a schematic of these influences are presented in Figure 1, these include genes associated with differences in nicotine's metabolic capacity and nicotine effects on central nervous system neurotransmitter functionality, specifically the dose that modulate direct and indirect effects on nAChR, dopaminergic and opioidergic activity, respectively