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Primary antibodies against HIF-1 (610958, BD Biosciences, Franklin Lakes, NJ), hydroxy-HIF-1 (3434, Cell Signaling Technology, Danvers, MA), HIF-2 (NB100-122, Novus Biologicals, Littleton, CO), TOM20 (11802-1-AP, Proteintech Group, Rosemont, IL), HSP60 (15282-1-AP, Proteintech Group), and Tubulin (sc-8035, Santa Cruz Biotechnology, Dallas, TX) were diluted in blocking buffer and incubated overnight at 4 C

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Tobacco companies have told ministers that the "tax clouds are gathering" as George Osborne prepares to push through a second year of above-inflation excise duty rises next month, on top of the already increased rate of VAT

This bacterium infects the lining of the stomach and small intestine, leading to inflammation and ulcer formation