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This leads to an increase in the activity of the glutathione-dependent enzymes, glutathione reductase and glutamate-cysteine ligase, and the glutamate/cystine antiporter, which are critical in the production of reduced glutathione, which plays a vital role in neutralizing ROS (Lee et al., 2003

Heart tissues isolated from FLAG-BNIP3 mice were initially washed with PBS, dissected, and homogenised in immunoprecipitation (IP) lysis buffer composed of 1 Cell Lysis Buffer (Cell Signaling), 100 mM PMSF (Roche), and 1 protease and phosphatase inhibitor cocktail (ThermoFisher)

Contemporary smart hydrogels go further by responding to multiple endogenous cues (enzymes, reactive oxygen species, pH, temperature) and exogenous cues (light, electricity, magnetism, ultrasound, mechanical force) with spatiotemporal control

& Suzuki, G