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(4) Diversity analysis: All samples of ASV were diluted to the same sequencing depth based on the sequencing depth of bacterial and fungal samples respectively, and then diversity and diversity analysis were performed ( 2.4 Analysis of volatile compounds in tobacco leaves Volatile compounds of tobacco leaves were determined by GC-MS (model 7890B-5977A, Agilent, Palo Alto, CA, United States) as follows: 0.5 g tobacco leaves powder was taken and 6 mL saturated NaCl solution was added

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