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Assays and ELISAs for lung proteins quantification We used the proteins extracted from the lung samples (as described above) to perform a series of ELISAs or enzyme activity assays to determine the lung concentrations or activity of specific proteins involved in iron metabolism [ferroportin-1 (LS-F7200-1), transferrin (LS-F9542-1), ferritin light chain (LS-F8829), all from LS Bio, Shirley, MA, and lactoferrin (A74883, from Antibodies, St Louis, MO)], antioxidant response (NQO1 activity, AB184867, Abcam, Waltham, MA), and airway mucus hypersecretion (MUC5AC, LS-F4842, LS Bio, Shirley, MA)

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Circa 1920s Size: 4 5/8" x 3" Weight: 130.1g Condition: shows signs of age and wear, including scratches, tarnish, and dents, monogrammed "H" on the front Available payment options In-house shipping is available for coins, jewelry, and sterling

[2 ] For every 1% increase in the number of families living below the poverty line, there was a .83% increase in tobacco retailer density for that community