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Then, this summary of a University of Washington study and the paper that documents the study took the next step

Many Native American communities, including hers, use the substance in traditional rituals and pass down stories about how and why the creator gave it to them

The resulting cDNA was amplified using 2 SYBR mix (Qiagen) and 1 M of each primer in a StepOne Plus Thermocycler (Applied Biosystems) in Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR)