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Despite significant research progress, key challenges and limitations persist

FMT in animal models has been found to reduce the abundance of the Akkermansia genus (in phylum Verrucomicrobia) and increase the abundance of the Prevotella genus (in phylum Bacteroidetes) in gut microbiota (Tankou et al., 2018b), which is in line with findings of decreased gut Akkermansia after probiotic interventions and increased gut Prevotella after first-line disease-modifying treatments and time-restricted eating in MS patients (Jangi et al., 2016

We also performed an immunofluorescence analysis for LC3 and observed that the number of LC3-labeled vacuoles formed increased upon glucose deprivation compared with that in cells cultured under glucose-replete conditions (Fig