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qPCR was performed using the TaqMan Fast Advanced Master Mix (Thermofisher) and the Taqman primers: H

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However, our findings suggest that there should also be thoughtful consideration to potential unintended consequences that could affect other subsets of users of e-cigarettes and other tobacco products. Pacek and other researchers at the Duke University Center for Addiction Science and Technology are continuing research on e-cigarette use, including an in-depth study tracking participants use of various tobacco products in response to potential regulations such as those posed by the online survey