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For instance, the Tar content per cigarette, nicotine levels, quality of the tobacco used, purity of the tobacco, type and quality of the paper employed in cigarette production, flavor and scent of the tobacco, size of the cigarette filter, and more

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After proper dilution of the TLC band extract with the mobile phase, 20 l of the diluted solution was injected to the HPLC column

*Correspondence: Harvey Ho [email protected] This article was submitted to Bioinformatics and Computational Biology, a section of the journal Frontiers in Bioengineering and Biotechnology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers