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Evaluations of Apoptosis, Cell Viability, and Caspase Levels A density of 2 10 3 sciatic nerve per milliliter was seeded in six-well plates with 100 l of MTT (5 mg/ml) in phosphate buffer solution

Cell supernatants were added to 96-well plates to determine LDH release, and absorbance was measured at 490 nm using an automated ELISA reader (SpectraMax M5, Molecular Devices, USA)

Conclusion The present investigation outlines coherently the different concentration levels of AITC and three mustard oils required to attain insect pest resistance against two diverse insect species belonging to different taxonomical Orders and their respective Families, reflecting an effective range of metabolites responsible over the Class Insecta

H.QingH.HilliardB.DlugosT.et al