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Quality control of each data was performed to filter out poor-quality cells based on numbers of genes per cell (nFeature), whole number of read counts (nCount) and the read counts percentage of mitochondrial genes per cell (percent_MT)

The PubMed search also revealed that the pioneering use of transferrin receptor ligands for brain-targeting nanoparticle drug delivery systems was reported in 1996 by Huwyler et al