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These framework substitutions are identified by methods well known in the art, e.g., by modeling of the interactions of the CDR and framework residues to identify framework residues important for antigen binding and sequence comparison to identify unusual framework residues at particular positions

For instance, in Alicyclobacillus acidoterrestris , The DnaK gene is induced to express under heat stress, which produces heat shock proteins (HSPs) to shield the microorganism from thermal damage

H., Zhang, Y., Zhang, X