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Functional signs that may suggest undermethylation or impaired methylation efficiency include: High internal tension Perfectionism, rumination, or OCD traits Chronic anxiety Low serotonin or low motivation Poor response to SSRIs Sensitivity to folate or methyl donors Increased inflammation Elevated homocysteine with normal or low B-vitamin status A methylation test that measures SAM, SAH, and homocysteine helps clarify the exact biochemical pattern

The new enzyme GST A3-3 was cloned from human placenta and characterized in our laboratory and found to be the most efficient ketosteroid isomerase so far detected in mammals ( Mechanism of Ketosteroid Isomerase Catalyzed by Mammalian Glutathione Transferases The double-bond isomerization of ketosteroids has been investigated with 5-AD and 5-PD (Figure 2)

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