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After 24 h, cells were treated with DMSO control or RSL3 (0.11.0 M) and incubated for 7 days to allow for tumor spheroid formation

This characteristic makes it particularly valuable for research investigating physiological growth hormone dynamics, as it stimulates pulsatile GH release that mimics the body's natural circadian and ultradian rhythms rather than providing sustained, non-physiological elevation

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