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Cycle threshold (Ct) values of target genes were calibrated to the Ct for the corresponding genes obtained for samples from the control pCYC1-GAP1 strain and normalised to the geometric mean of the reference genes for relative quantification using the comparative Ct method [36]

B Vitamins (B1, B5, B12) The B vitamin family plays a central role in energy metabolism and nervous system health

In addition, we found almost no change of OLFM4 level in MVs when the secretion of exosomes was blocked by N-SMase inhibitor GW4869, confirming MV-specific secretion of OLFM4 (Supplementary Fig