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To incorporate such in silico predictions in a comprehensive carrier design framework, one needs: Direct engagement of target validation (competitive binding assays, cellular thermal shift assays, or kinase activity) for confirmation that naringenin effectively engages these targets at therapeutically achievable concentrations in vivo

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Functional metabolic screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 as an important regulator of prostate cancer cell survival

Additionally, research has demonstrated that probiotic-treated larvae exhibit increased glp-1 gene expression, potentially due to the metabolic activity of lactic acid bacteria producing SCFAs (Falcinelli et al., 2016)