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BSO was inoculated intraperitoneally 5 h before cisplatin or TMZ treatment for three consecutive days and then once a week for 2 weeks

Massage gently in the morning and evening

At the same time, N-cadherin and -SMA expression by AS-IV in podocytes treated with different HG concentrations are reversed under the mediation of the autophagy inhibitor 3-methyladenine, the activation of E-cadherin and nephrin levels and the inhibition of TGF-

For mitolysosome quantification with pimonidazole staining, images were taken at 63 magnification on a Zeiss LSM 880 confocal with 12 images total per eye from each group (24 images per mouse)