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Petersen KF, Dufour S, Savage DB, Bilz S, Solomon G, et al

Deproteinization efficiency was determined by measuring the protein content in each extract aliquot prior to the 5-fold dilution with acetonitrile

Hypochlorite leads to chlorination of the thiol group to form sulfenylchloride that is unstable and rapidly reacts further to form mixed BSH protein disulfides (Hawkins et al., 2003