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Antioxidant enzymes and peroxidation in liver tissue To assess oxidative damage and the effects of the test drug, liver tissue samples were homogenized in 0.1 M sodium phosphate buffer (pH 7.4) containing phenylmethylsulfonyl fluoride (PMSF) and then centrifuged at 4,000 g for 10 min at 5C

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As a result, fewer MRI assessments were required to fully enroll the study, reducing costs and the need for patients to be scheduled for a separate imaging visit