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The concentration of the extracted RNA was measured with a NanoDrop One spectrophotometer (ThermoFisher)

Linarin alleviates high-fat diet-induced NAFLD via modulating the PI3K/Akt/mTOR pathway, autophagy, and gut microbiota

PARP-mediated ribosylation activity is detected by adding Streptavidin-HRP (horseradish peroxidase), which binds to the newly formed ribosylation branch, and a chemiluminescent or colorimetric HRP substrate