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2.3.1 DNA methylation profiling, psoriasis versus controls Methylation profiles of CD4 + T-cells were analyzed using R packages minfi (20) and ChAMP (21)

To resolve the phylogenetic relationship of TyrA orthologs from divergent organisms including plants and microbes, here we performed structure-guided phylogenetic analyses using PROMALS3D to achieve alignment of TyrA orthologs with low sequence similarities (see section Materials and Methods

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681 Consequently, the application of CRISPR/Cas9 technology to mtDNA manipulation remains highly challenging and will require further extensive research to overcome these obstacles